experimental study for evaluating the efficacy of cercarial vaccine against Schistosoma mansoni

This study assessed the effectiveness of autoclaved cercarial vaccine [ACV] in protection against Schistosoma mansoni infection in 125 Swiss albino mice classified into two main groups: GI: a control group. Gil: a test vaccinated with ACV, in a single dose of 0.1ml of 10[4] ml ACV [G.IIa], double dose; 0.2ml [G.IIb] and two single doses 2 weeks apart [G.IIc]. Four weeks later, all mice were challenged with S. mansoni cercariae and sacrificed 10 weeks post infection [P.I.]. The results revealed that the vaccine in a single dose [G.IIa] induced a high level of protection against S. mansoni infection. There was a significant reduction in the mean number of adult worm [91.12%], ova/gram liver [91.87%], ova/gram intestine [89.09%] and number and size of granulomas in liver [92.92% and 43.53% respectively]. Besides, ACV induced a significant increase in the level of IL-10 mRNA expression as compared to the control group

seroepidemiologic study of strongyloids stercoralis infection in Shoubra El-Namla village, Gharbyia governorate, Egypt

An epidemiologic survey for Strongyloides stercoralis infection among primary school children was conducted in an Egyptian village in Gharbia Governorate using agar plate culture [APC] of stool, and Enzyme-linked Immunosorbent Assay [ELISA] technique. Out of the 1083 studied children, 53 [4.89%] were detected by APC. All were confirmed serologically by ELISA. An additional 34 cases were detected by ELISA giving a total of 87 [8.03%] seropositive children. Cases were evaluated for predisposing factors, clinical manifestations and treatment outcome [using ivermectin in a single dose of 200 mug/kg]. The obtained laboratory data, epidemiologic features and clinical manifestations were presented. A cure rate of 83.33% was obtained with ivermectin

Hepatic changes in experimental trichilemma spiralis infection

Histopathological and immunofluorescent studies were carried out on the liver in rats experimentally infected with Trichinella spiralis throughout 24 weeks after infection. Definite histopathological changes were found in the liver of infected animals from the early weeks after infection in comparison with the control animals. The deposition of specific antigens and immune complexes was demonstrated in the infected animal liver by means of direct and indirect immunofluorescence. Few larvae were detected in the liver of infected animals. The findings were discussed and the role of immune complex deposition in the pathogenesis of the hepatic lesions was declared

histopathological study of different clinical forms of cutaneous leishmaniasis

Biopsy specimens for histopathological study were taken from 15 parasitologically proven cases with different clinical forms of cutaneous leishmaniasis [CL] including two patients with disseminated CL in the form of subcutaneous nodules and satellite papules. The clinical picture of these patients was also reported. The study revealed that the histopathological manifestations were variable, ranging from a diffuse infiltrate in the acute stage to a tuberculoid architecture in the chronic stage. One patient had perineural inflammatory cell infiltrate consisting of lymphocytes, plasma cells and macrophages

Childhood scabies: what is new in the old problems?

This study aimed to investigate the problem of scabies among pupils enrolled in primary schools in Nawag Village, Gharbia governorate. Out of 1337 pupils examined, 97 were confirmed as cases of scabies with a prevalence rate of 7.3%. Boys were more affected than girls [8.2% and 6.4%, respectively]. Other than Sarcoptes scabiei. Dermanyssus gallinae or Red Poultry Mite was isolated from one pupil and other members of his family. Scabies was found to be mainly transmitted outside schools. Close personal contact as well as infested finites could have been routes of scabies transmission

Isolation of soil amoebae from the nasal mucosa of children in Tanta - Egypt

Forty children attending the outpatient clinic, Tanta University Hospitals were examined by nasal swabing in order to discover the healthy free-living amoebae carriers. The swabs were cultured on non-nutrient agar with active E-coli and incubated at 37°C and 45°C. Two species of free-living amoebae were identified: Hartmannella agricola and Acanthamoeba polyphaga. Pathogenicity of these amoebae was tested by intranasal instillation in mice. During the observation period of two months, 20% of mice died within 21 days post inoculation. The possible role of these amoebae in the nasal cavity and the way of contamination are discussed